Fig. 3. AOF induces apoptosis in oral cancer cells. (A) Cells were treated with various concentration of AOF 0.2% for 48 hr in YD-8 and YD-10B cells. Western blot analysis was used to analyze apoptosis-related factor protein expression. (B, C) Oral cancer cells were treated with indicated concentration of AOF for 48 hr. Apoptotic cells were stained with annexin V and PI and analyzed by Arthur image based cytometry. Data are expressed as mean±standard deviations. The Y-axis represents the percent of cells positive for annexin V (green), PI (red), annexin V and PI (yellow), or negative for annexin V and PI (blue). AOF, Aspergillus oryzae fermentation extract; PI, Propidium Iodide; PARP, poly-ADP ribose polymerase. **p< 0.01, ***p< 0.001 vs. control (comparison of viable cells).
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