Oral Biol Res 2019; 43(1): 17-22  https://doi.org/10.21851/obr.43.01.201903.17
The effect of cigarette smoking on Porphyromonas gingivalis, a crucial periodontal pathogen
Junghoon Shin1† , Seok-Woo Lee2 ,3*
1Department of Dental Science, School of Dentistry, Chonnam National University, Gwangju, Korea
2Department of Dental Education, School of Dentistry, Chonnam National University, Gwangju, Korea
3Department of Periodontology, School of Dentistry, Chonnam National University, Gwangju, Korea
Correspondence to: Seok-Woo Lee, Departments of Dental Education and Periodontology, School of Dentistry, Chonnam National University, 33 Yongbong-ro, Buk-gu, Gwangju 61186, Korea. Tel: +82-62-530-5820, Fax: +82-62-530-5810, E-mail: swlee@chonnam.ac.kr
Received: October 18, 2018; Revised: December 13, 2018; Accepted: December 17, 2018; Published online: March 31, 2019.
© Oral Biology Research. All rights reserved.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Smoking is implicated as one of the most significant risk factors for periodontal disease. The objective of this study was to examine the effect of cigarette smoke extract (CSE), on cultivation of and protein production by Porphyromonas gingivalis, a major periodontal pathogen. P. gingivalis were cultivated in the presence of CSE (0%, 2.5%, 5%, and 10%) solution, and their growth was measured by optical density. In addition, expression pattern of proteins produced by P. gingivalis under CSE influence, was examined by sodium-dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). According to the results, the growth of P. gingivalis was inhibited by CSE in dose-dependent mode. SDS-PAGE analyses revealed minor difference in expression pattern of proteins. Results of this study suggest that CSE imposes an inhibitory influence on growth of P. gingivalis, and is capable of modulating protein expression in P. gingivalis.
Keywords: Microbiology, Porphyromonas gingivalis, Smoking, Virulence


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Funding Information
  • Chonnam National University Hospital, Research Institute of Clinical Medicine
      10.13039/501100002456
      CRI 17025-1

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